Lab Manager

OBJECTIVE:
To obtain a senior level position within the corporate community that utilizes my experience and education.

QUALIFICATIONS:
A Masters degree with over five years of managerial responsibilities in a laboratory setting, along with over five years of scientific experience.

EMPLOYMENT HISTORY:
(Lab Manager) Washington University School of Medicine, St. Louis, MO
Division of Bone Marrow Transplant/Stem Cell Biology
November ••••• to Present

(Lab Manager) Washington University School of Medicine, St. Louis, MO
Department of Molecular Biology & Pharmacology
May l••••• to November •••••

(Technician) Washington University School of Medicine, St. Louis, MO
Division of Internal Medicine/ Nephrology
Chromalloy American Kidney Center
April l••••• to April l•••••

(Technician) Austin Diagnostic Clinic, Austin, TX
Division of Internal Medicine/ Nephrology
June l••••• to November l•••••

RESEARCH:
Mechanisms of Stem Cell Mobilization and Proliferation
Homologous Recombination as a tool for correcting gene mutations
Washington University School of Medicine, Bone Marrow Transplant/Stem Cell Biology
Principal Investigator- Dr. Tim Ley

Mechanism of Signal Transduction in the Notch Signaling Pathways
Washington University School of Medicine, Molecular Biology & Pharmacology
Principal Investigator- Dr. Raphael Kopan

Stenosis and Restenosis of Dialysis Vascular Access
Washington University School of Medicine, Division of Internal Medicine/ Nephrology
Principal Investigator- Dr. David Windus

Meadox Flow Study of Gortex Grafts/ Pharmacology Study
Meadox Inc., Boston, MA
••••• NOR Study, Vitamin D
Washington University School of Medicine, Division of Internal Medicine/
Principal Investigator- Dr. David Windus

PRINCIPLE DUTIES & RESPONSIBILITIES:
MANAGERIAL:
Maintained budgets for the fiscal year
Responsible for all aspects of personnel schedules
Trained and supervised personnel
Responsible for all purchases
Maintained various computer databases for laboratory use
Presented updates on given topics
Updated and Maintained laboratory specific equipment
Assured compliance with safety regulations
Maintained data files
Monitored data to ensure consistent quality
Wrote and edited reports
Teaches others inside/outside the lab about proper methods and procedures
Conducts literature reviews for projects
Prepared and organized DNA and cell lines for laboratory use
Managed set-up and maintained computer LAN network

SCIENTIFIC:
Project design and set up of experiments
Prepared data and presented reports on ongoing research
Maintained required documentation on laboratory and research specimen conditions
Responsible for generation and transfection of Mouse Embryonic Stem Cells for gene therapy studies
Worked with lentivirus as a mode of delivery in Embryonic Stem cell gene therapy studies
Worked with multiple mammalian cell culture lines
Worked with Primary Bone Marrow cells
Flow Cytometry used for immunology cells and transfected cell detection
Performed purification assays
Bacterial plating
Antibiotic testing of bacteria
Numerous Molecular Biology experiments
Performed morula aggregation on mouse embryos
Microinjections of mouse blastocysts
Performed surgical implantation of mouse embryos
Surgical removal and analysis of tissue collection (mouse)
Mouse Intraperitoneal injections
Genotyping of mice by PCR
Updating myself on relevant scientific literature and scientific techniques
Co-implemented vascular flow study
Performed laptop PC studies of patient access flows
Monitored patient blood chemistry and vital signs

PUBLICATIONS:
Zhi Hong Lu, ••••• T. •••••, Richard M. Kaufman, and Timothy J. Ley. Correction of a Mutant Murine beta-globin Gene by Homologous Recombination in Embryonic Stem Cells: Very Long Targeting Arms Do not Increase Efficiency. Blood. ••••• May 1

Kopan R, Lee J, Lin MH, Syder AJ, Kesterson J, Crutchfield N, Li CR, Wu W, ••••• J, Gordon JI. Genetic mosaic analysis indicates that the bulb region of coat hair follicles contains a resident population of several active multipotent epithelial lineage progenitors. Dev Biol ••••• Feb 1;•••••(1):•••••



EDUCATION:
Washington University in St. Louis, May •••••, Masters in Biology
Southern Illinois University at Edwardsville, Bachelor of Science/Biology
Texas Tech University, Lubbock, TX.
Rend Lake College, Ina, IL- Associate Degree in Science

REFERENCES:
Available Upon Request