An accomplished, dedicated PhD with a proven background in biomedical research work, currently seeking a position that requires expertise in Cell Biology/immunology/molecular biology /life sciences.
••••• PhD (Molecular Biology and Immunology) in the Department of Biotechnology, All India Institute of Medical sciences (AIIMS), New Delhi- India. (Viva held on •••••)
••••• M. Sc. (Biotechnology) Pantnagar university, Uttranchal, India, (•••••%).
••••• B. Sc. Rohilkhand Univ. Bareilly (U.P.), India, (•••••%).
••••• Intermediate, Prabha Devi Inter College (U.P. Board), India, (•••••%)
••••• High School, Prabha Devi Inter College (U.P. Board), India, (•••••%)
•••••: Successfully completed the course on Biostatistics and Research Methodology during Ph.d.
Research Positions and Experience:
•••••: Cleared the prestigious National level Fellowship of All India Institute of Medical Sciences, (AIIMS), New Delhi.
•••••: Junior Research Fellow from Indian Council of Medical Research, India.
•••••: Senior Research Fellow from Indian Council of Medical Research, India
••••• •••••February: Post Doctoral Fellow, Department of Cancer Biology, College of Medicine, Peoria, IL.
••••• February-present: Post Doctoral Fellow, Department of Chemistry-Biomed, University du Quebec a Montreal, Canada.
•••••: One and half year experience while teaching IMMUNOLOGY, Recombinant DNA technology, structural Biology and Animal Cell
Biology to ••••• and ••••• students in Amity University, Noida, India.
•••••: One-year research project in RAPD Profiling of small grained Indian Aromatic Rice in the Department of Molecular Biology and Genetic Engineering, ••••• University, Pantnagar, India.
••••• Best scientific model award
••••• GATE qualified (All India •••••th rank)
••••• ICMR qualified (All India •••••th rank)
••••• Best Feedback award in teaching
American Association of Cancer Research (AACR).
(Membership number: •••••)
Member: Society for Neuroscience (Membership number: •••••)
Reviewer and Editorial Board member for ANSI journals: Journal of Biological Sciences
Personal Skills and Competencies:
Oversaw the management of summer intern research students and co-mentored their projects.
Worked as a part of highly motivated teams, assisted in execution of short term projects for rotation students during teaching.
Mentor of Biotechnology Programme while teaching.
Gene cloning and expression, RNA isolation, Immunoprecipitation, Isolation, purification and quantitation of plasmid DNA and genomic DNA (CsCl2 density
gradient centrifugation and phenol-chloroform extraction), Restriction enzyme digestion of plasmid DNA and genomic DNA, Southern Blotting of restricted enzyme treated plasmid DNA, Apoptotic gel preparation (Apoptosis caused by Mtx and 5 FU has been seen by this method), PCR, RT-PCR, Real time PCR, RAPD (Random amplified polymorphic DNA), DNA sequencing (TNF sequencing by automated sequencer), RFLP (Restriction fragment length polymorphism), SDS-PAGE, Native PAGE.
Sandwitch ELISA, Western Blotting, Nitric oxide estimation by Greis reagent method, Immunohistochemistry, Lymphocyte proliferation test, IL••••• assay, Indirect Immunofluorescence (IFA) (CD4, CD8 and CD3), Sample acquiring and FACS analysis, Dot ELISA, IgG Purification, RIA, Immnunoelectrophoresis, Single Radioimmunodiffusion assay, Ouchterlony, Antibody purification by affinity chromatography, Widal test, Agglutination test, ELISPOT
Kinase assay, Electrophoretic mobility shift assay, Supershift assay, Primary cell culture and cell line maintenance, matrigel invasion, spheroid migration assay, angiogenesis, MTT, Tunel, DNA Fragmentation, Annexin V binding assay
SPSS, Sigma Plot •••••, Multivariate statistics, Experience in data acquisition, analysis and presentation.
Proficient in use of MS Office, Photoshop and research data analyses softwares
Have gained the experience of working on nude mice for:
a) Dorsal skin chamber assay for angiogenesis using medulloblastoma cells
b) Intracranial injection of meningioma cells and establishment of orthotopic tumor models
Conferences Attended and Abstracts:
&#•••••; XXI Annual Convention of the Indian Association for Cancer Research, Indian Institute of Science, Bangalore, January •••••February 2, •••••
&#•••••; International Conference on Emerging Trends in Cancer Research, JNU, Delhi, ••••• March •••••
&#•••••; International symposium on Cancer and Cell Death, NCBS, Bangalore, (January •••••, •••••)
&#•••••; •••••nd Annual convention of the Indian Association for Cancer Research and International symposium on recent advances in cancer causes and control Regional Cancer Centre, Thiruvananthapuram, January •••••, •••••
&#•••••; Workshop on Cervical cancer, National Centre for Biological Sciences, Bangalore, August •••••, •••••
&#•••••; International Workshop on Cervical Cancer, NCBS, Bangalore, November •••••, •••••
&#•••••; Participated and presented the abstract with poster TNF&#•••••;, TNF R1 and TNF RII gene polymorphism in Indian oral cancer patients in the International Symposium on Diet in Causation & Prevention of Cancer and XXXth Annual Conference of Environmental Mutagen Society of India, Lucknow, March •••••, •••••
&#•••••; Downregulation of uPA/uPAR Inhibits Epithelial to Mesenchymal Transition (EMT) in Intermittent Hypoxic DAOY and D••••• Medulloblastoma Cells. AACR, •••••th •••••th April •••••
&#•••••; Radiation inducible silencing of uPA and uPAR in vitro and in vivo in meningioma. AACR, •••••th •••••th April •••••
&#•••••; Recruitment of endothelial cells by radiation induced monocyte chemoattract protein••••• in meningioma is blocked by suppression of urokinase plasminogen activator, AACR.
••••• •••••, R., Sharma, SC., Das, SN. Association of polymorphisms in TNF-&#•••••;, TNFR1 promoters and 3 UTR region of TNFR2 gene in Asian Indians with tobacco related Oral Carcinoma. Oral Oncology: ••••• May; •••••(5):•••••
••••• ••••• R, Kapuria V, Das SN. Single Nucleotide Polymorphism in TNF-&#•••••;, TNFR2 gene and TNF-&#•••••; production in Asian Indians. (Immunoligcal Investigations Immunol Invest. •••••;•••••(•••••):•••••).
••••• ••••• R, Chandramu Chetty, Praveen Bhoopathi, Sajani Lakka,Jasti S Rao, Dzung H. Dinh. Downregulation of uPA/uPAR inhibits epithelial-mesenchymal transition (EMT) in intermittent hypoxic DAOY and D••••• medulloblastoma cells (Int J Oncol. ••••• Dec ••••• doi: •••••/ijo•••••).
••••• Venkateswara Rao Gogineni, Arun Kumar Nalla, ••••• •••••••••• Bharathi Gorantla, Meena Gujrati, Dzung H. Dinh Daniel Fasset, Jeffrey D. Klopfenstein and Jasti S. Rao. Radiation inducible silencing of uPA and uPAR in vitro and in vivo in meningioma. International Journal of Oncology, Apr; •••••(4):••••• •••••).
••••• Ganji, P.N., Nalla, A.K., •••••, R., Mohanam, S., Gujrati, M., Dinh, D.H., Rao, J.S. siRNA-mediated downregulation of MMP••••• and uPAR in combination with radiation induces G2/M cell cycle arrest in medulloblastoma. (Mol Cancer Res. ••••• Jan;9(1):••••• Epub ••••• Dec •••••).
••••• ••••• R, Gogigeni V, Nalla AK, Daniel Fassett, Jeffrey D. Klopfenstein, Dzung H. Dinh, Meena Gujrati, Rao JS. Oncogenic role of p••••• is suppressed by si-RNA bicistronic construct of uPA, uPAR and cathepsin-B in meningiomas both in vivo and in vitro (Int J Oncol. ••••• Feb ••••• doi: •••••/ijo•••••). ••••• ••••• R, Nalla AK, Gogigeni V, Chandramu Chetty, Praveen Bhoopathi, Dzung H. Dinh, Meena Gujrati, Rao JS. Reversibility of angiogenesis by uPAR/ cathepsin B overexpression in pUC transfected meningioma cells by rescuing Y••••• FAK phosphorylation both in vitro and in vivo (PLoS ONE ••••• Feb •••••;6(2):e•••••).
••••• Nalla, A.K. Gogineni, V.R., •••••, R., Dinh, D.H., Rao, J.S. Suppression of uPA and uPAR blocks radiation-induced MCP••••• mediated recruitment of endothelial cells and monocytes in meningioma. Cell Signal. ••••• Mar •••••
••••• Venkateswara Rao Gogineni, Arun Kumar Nalla, ••••• ••••• and jasti S. &#•••••;3 &#•••••;1 Integrin Promote Radiation-induced Migration of Meningioma cells (Int J Oncol. ••••• Mar ••••• doi: •••••/ijo•••••).
••••• ••••• R, Sharma SC, Das SN. TNF, TNFR2, NF-kB signaling in Oral Cancer (Communicated Oral Diseases).
••••• Gogigeni V, Nalla AK, ••••• R, Daniel Fassett, Jeffrey D. Klopfenstein, Dzung H. Dinh, Meena Gujrati, Rao JS Chk•••••Mediated G2/M Cell Cycle Arrest Maintains Radiation Resistance in Malignant Meningioma cells (Cancer Letters).
••••• ••••• R, Nalla AK, Gogigeni V, Chandramu Chetty, Praveen Bhoopathi, Dzung H. Dinh, Meena Gujrati, Rao JS. Transforming Growth Factor &#•••••; increases cellular proliferation of meningioma carcinoma cells through Phosphatidylinositol 3 kinase and MEK dependent upregulation of X-linked inhibitor of apoptosis (XIAP) (Manuscript under preparation).
Canada Visa: Work Permit
USA Visa: H1B
UK Visa Status: Dependent visa (Tier one)
Languages known: English, Hindi