Research Scientist

Education:

Degree of Medicine, Zhong Shan University (formerly Sun Yat-Sen University of Medical Sciences, SUMS), Guangzhou, •••••a. •••••, in Immunotoxicology, Zhong Shan University, •••••a

Work Experience:

••••• •••••: Associate Scientist III, GlaxoSmithK•••••ne Biologicals North America, Laval R & D Center, Quebec, Canada. Working on a GLP comp•••••ant program to support Flu Pandemic/seasonal vaccine projects since mid ••••• In the last few years, also worked on relevant Influenza vaccine studies, Allergy vaccine study, Oncology project and the Plague-DVC-CMI project. Responsible for study/experimental design, sample testing, data analysis, and report writing. All projects were relating to in vivo and in vitro immunological/virology studies including virus culture, titration, amp•••••fication and challenge in various animal models; Myeloma cell •••••ne/Hybridoma culture, Cell fusion/Monoclonal antibody production, intracellular cytokine detection, lymphocyte pro•••••feration assay, DTH experiments, E•••••SA, and HAI assay, etc.

•••••: Researcher Scientist, Charles River Lab Research (formerly CTBR Bio Research Inc. a CRO in prec•••••nical studies for drug development under a GLP comp•••••ant environment). Senneville, Quebec, Canada. Worked on immunotoxicology studies, managed projects as Study Director. Responsible for study/experimental design, sample testing, data analysis, and report writing including detection of antibody/cytokines, lymphocyte pro•••••feration, antibody production, etc.). All projects were performed under guidance of FDA, ICH, EPA, OECD or Japan MHW and GLP regulations.

•••••: Research Associate, Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada. In reproductive Toxicology, performed two TSRI (Toxic Substance Research Initiative, Health Canada) grants in Reproductive/Developmental toxicology. Responsible for study design, conduct, data analysis & interpretation, and pub•••••cations prepared. Worked with 8 doctors as a researcher and project coordinator.

•••••: Visiting Scientist, Wadsworth Center, New York State Department of Health /School of Pub•••••c Health, State University of New York at Albany, NY, USA. In Molecular Immunotoxicology/Cell Biology, conducted a NIH grant in effects of heavy Metals/PCBs on Apoptosis & Oxidative Stress in lymphocytes/Th1 & Th2 clone cells, and an EPA grant in assessment of developmental toxicity in the rat.

•••••: Visiting Scientist, Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore. In Immunotoxicology, ran a CMB (China Medical Board, headquarter in New York, USA) project in effect of nickel chloride on lymphocyte subsets in mouse.

•••••: Visiting Scientist, Department of Pub•••••c Health, Sapporo Medical University, Sapporo, Japan. In Environmental toxicology, obtained training in effects of heavy metals (e.g. Cadmium) on biological systems in mouse.

•••••: Faculty, Department of Environmental Health, SUMS, Guangzhou, •••••a. Responsible for teaching and scientific research in Immunology/Toxicology, Environmental Health, and human health risk assessment.

Professional Membership:

Full member, Society of Toxicology (SOT, USA)
Regular member, Society of Toxicology of Canada (STC)
Canadian Federation of Biological Societies (CFBS)

Areas of Knowledge & Expertise:

General: knowledge and experience in Medical Sciences, Immunology (refreshed my knowledge by attending Introductory Course in Immunology on June ••••• •••••, ••••• from AAI American Association of Immunologists, USA), Toxicology, Pharmacology, Molecular biology/cell biology, Genetics, etc.; Team play experience (e.g. at GSK Bio, McGill university, CTBR, etc.)

Immunology/Virology: virus culture, titration, amp•••••fication and challenge in various animal models; various cell culture (SP2/0 A••••• Myeloma cell •••••ne, hybridomas, PC••••• cells, Human mast cell •••••ne LAD2, THP••••• cells, Th1/Th2 clone cells, splenocytes, thymocytes, NK cells, etc.), cell fusion, monoclonal antibody production, Developmental Immunotoxicology (DIT), host resistance model, E•••••SA/EIA, RIA, cytotoxicity assay (NK cell activity), DTH, lymphocyte pro•••••feration assays, etc.

Toxicology: study for acute toxicity (LD•••••/LC•••••), subacute toxicity, chronic toxicity; Reproductive toxicity; immunotoxicity; Genetic toxicity; Including study design, study performance, data analysis/interpretation, and report/pub•••••cation preparation. Up to •••••+ years of so•••••d experiences in the area of Immunotoxicology.

Genetics: knowledge in medical genetics (an entry examination for MSc program). Obtained training in regular genetics techniques, which included SCE, Micronuclei Test, and Chromosome Abnorma•••••ty Test.

Animal study: various injections, gavage, organ perfusion (e.g. reproductive system, lung, etc.), blood/tissue samp•••••ng, surgery (e.g. bile duct, brain, etc.), necropsies, etc.

Molecular Biology: DNA/RNA extraction, Atlas cDNA expression array (gene expression), PCR, Western blotting

Others: metallothionein (MT) purification from rat •••••ver; Protein detection (Lowrys, BCA assay), Basic HPLC; Atomic Absorption spectrometer (AAS) for heavy metals; Detection of GSH, Ca2+, ROS, Mitochondrial function by Flow cytometry, App•••••cation of the MicroBeta counter and Gamma counter for immunology assays using radioactive, etc.

Statistical analysis: took course in this area about ••••• hrs, can perform various data analysis or prepare graphs using SigmaStat/SigmaPlot, Excel and Prism.

Regulatory: fami•••••arity with international regulatory guidances in vaccine /immunology /toxicology studies. (e.g. FDA, ICH, OECD, EPA, MHW/Japan etc) Representative Pub•••••cations:
•••••a Kishta , Adedayo Adeeko, ••••• •••••, Trang Luu, Louis Hermo, Carlos Morales, Daniel G. Cyr, James Brawer, Jacquetta, M. Trasler, Bernard Robaire and Barbara F. Hales. In utero exposure to tributyltin chloride differentially alters male and female fetal gonad morphology and gene expression profiles in the Sprague-Dawley Rat. Reproductive Toxicology. •••••; •••••:1 - ••••• •••••n •••••al, ••••• •••••, Kamal Swami, J. Kenneth Dean, Charles Hauer, and David Lawrence. Mercury impairment of mouse thymocytes survival in vitro: involvement of cellular thiols. J. Toxicol Environ. Heal.A . •••••;•••••(7):••••• •••••sheng Tan, ••••• •••••, Renjie Song, David Lawrence, and David O. Carpenter: Ortho-Substituted PCBs Kill Thymocytes . Toxicol. Sci. •••••; •••••: •••••ayo Adeeko, ••••• •••••, Don S. Forsyth, V. Casey, Johanna Barthelemy, Daniel G. Cyr, Jacquetta, M. Trasler, Bernard Robaire and Barbara F. Hales. Effects of in Utero Tributyltin Chloride Exposure in the Rat on Pregnancy Outcome. Toxicol. Sci. •••••; •••••: •••••
•••••ayo Adeeko, ••••• •••••, Josee Doucet, Gerard M. Cooke, Jacquetta, M. Trasler, Barbara F. Hales and Bernard Robaire. Gestational Exposure to Persistent Organic pollutants: Maternal •••••ver Residues, Pregnancy Outcome, and Effects on Hepatic Gene Expression Profiles in the Dams and Fetus. Toxicol. Sci. •••••; •••••:•••••

••••• D, Ong CN, NG V, Tan C. Effects of nickel chloride on mouse T-Lymphocyte ••••• Hum Toxicol. ••••• Jun; •••••

References: Available upon requested